1. Me pehea te whakamahi i te whakamaarama mamaoa pehanga teitei
1. Tāpirihia te wai ki te taumata wai o te autoclave i mua i te whakamahi;
2. Whakanohoia te tikanga tikanga, te wai matutu, etahi atu taputapu ranei e tika ana kia whakahoroa ki roto i te kohua whakahoromata, kati te taupoki o te kohua, ka tirohia te mana o te puera rewharewha me te paera haumaru;
3. Tahurihia te mana, tirohia mehemea he tika nga tautuhinga tawhā, ka pehi i te paatene "mahi", ka timata te mahi a te sterilizer; ka tukuna aunoatia te hau matao ki te 105°C, ka kati aunoa te reera o raro, katahi ka timata te pehanga ki te piki;
4. Ka piki ake te pehanga ki te 0.15MPa (121°C), ka mimiti ano te kohua whakahoromata, katahi ka timata te wa. Ko te tikanga, ka whakahoroa te tikanga tikanga mo te 20 meneti, ka whakahoroa te wai makuku mo te 30 meneti;
5. I muri i te taenga ki te wa whakahoromata kua tohua, whakawetohia te hiko, whakatuwhera i te puera hau kia puhoi haere; ka taka te tohu tohu pehanga ki te 0.00MPa karekau he mamaoa i puta mai i te puera hau, ka taea te whakatuwhera i te taupoki kohua.
2. Whakatupatotanga mo te whakamahi i nga whakamaarama mamaoa pehanga teitei
1. Tirohia te taumata wai i raro o te whakahoromata mamaoa hei aukati i te pehanga teitei ina he iti rawa te wai i roto i te kohua;
2. Kaua e whakamahi i te wai tap hei aukati i te waikura o roto;
3. I te wa e whakakiia ana te wai i roto i te kaipahua pehanga, wetekina te waha pounamu;
4. Ko nga mea hei whakahoroa me takai kia kore e marara ki roto, kia kaua e piri rawa;
5. Ina he tiketike rawa te pāmahana, kaua e whakatuwherahia, kaua e pa atu kia kore e wera;
6. I muri i te whakahoromatatanga, ka whakahekehia, ka whakahekehia te BAK, ki te kore ka koropuke te wai o te pounamu, ka peia te puru ka puhake, ka pakaru ranei te ipu. Ka taea anake te whakatuwhera i te taupoki i muri i te hekenga o te pehanga o roto o te whakahoromata kia rite ki te pehanga hau;
7. Tangohia nga mea kua whakahoroa i roto i te waa kia kore e mau ki roto i te kohua mo te wa roa.